TLS Online TPP Program

#Question id: 16030


Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

#Section 6: Plant, Animal and Microbial Biotechnology
  1. established cell lines
  2. primary cell lines
  3. secondary cell lines
  4. propagated cell lines
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TLS Online TPP Program

#Question id: 14245

#Section 5: Bioprocess Engineering and Process Biotechnology

Ethanol is produced by anaerobic fermentation of glucose by Saccharomyces cerevisiae. For the particular strain of S. cerevisiae employed, the maintenance coefficient is 0.18 kg kg- 1 h- 1, Yxs is 0.11 kg kg-1, YPX is 3.9 kg kg-1 and µmax is 0.4 h-1.  It is decided to investigate the possibility of using Zymomonas mobilis bacteria instead of yeast for making ethanol. Z. mobilis is known to produce ethanol under anaerobic conditions using a different metabolic pathway to that employed by yeast. Typical values of Yxs are lower than for yeast at about 0.06 kg kg-1; on the other hand, the maintenance coefficient is higher at 2.2 kg kg -1 h -1. Ypx for Z. mobilis is 7.7 kg kg-l; µmax is 0.3 h- 1. Y’PS is maximum and equal to the theoretical yield when there is zero growth and all substrate entering the cell is used for maintenance activities. If ethanol is the sole extracellular product of energy-yielding metabolism, calculate mp for yeast?

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TLS Online TPP Program

#Question id: 4141

#Section 3: Genetics, Cellular and Molecular Biology

Bacterial ribosomes:

TLS Online TPP Program

#Question id: 3659

#Section 3: Genetics, Cellular and Molecular Biology

In eukaryotes, gap filling in BER involves predominantly DNA polymerase β whereas the longer gaps generated in NER are filled by:

TLS Online TPP Program

#Question id: 2016

#Section 2: General Biology

Most newly emerging diseases, no matter how severe their effects on a population, human or otherwise, have which of the following in common?

TLS Online TPP Program

#Question id: 3687

#Section 3: Genetics, Cellular and Molecular Biology

As the two strands of the double helix are separated, the positive supercoiling interferes with the further unwinding of DNA. Which of the following enzyme makes a break in a strand of DNA to release the supercoiling and facilitate the replication to occur?