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#Question id: 14314

#Section 5: Bioprocess Engineering and Process Biotechnology

An industrial waste-water stream is fed to a stirred-tank reactor continuously and the cells are recycled back to the reactor from the bottom of the sedimentation tank placed after the reactor. The following are given for the system: F = 100 l/h; S0 = 5000 mg/l; mm = 0.25 h^-1 ; Ks = 200 mg/l; α (recycle ratio) = 0.6; C (cell concentration factor) = 2; Y ^M _X/S = 0.4. The effluent concentration is desired to be 100 mg/l.  The effluent concentration is desired to be 100 mg/l. If the residence time is 2 h in the sedimentation tank, determine the volume of the sedimentation tank and cell concentration in the effluent of the sedimentation tank.

#Question id: 14315

#Section 5: Bioprocess Engineering and Process Biotechnology

Anaerobic digestion of volatile acids by methane bacteria is represented by the equation:

The composition of methane bacteria is approximated by the empirical formula CH_1.4O_o.40N_0.20. For each kg acetic acid consumed, 0.67 kg CO ₂ is evolved. How does the yield of methane under these conditions compare with the maximum possible yield?

#Question id: 14316

#Section 5: Bioprocess Engineering and Process Biotechnology

Cellulomonas bacteria used as single-cell protein for human or animal food are produced from glucose under anaerobic conditions. All carbon in the substrate is converted into biomass; ammonia is used as nitrogen source. The molecular formula for the biomass is CH_I.560_0.54N_0.16; the cells also contain 5% ash. How does the yield of biomass from substrate in mass and molar terms compare with the maximum possible biomass yield?

#Question id: 14317

#Section 5: Bioprocess Engineering and Process Biotechnology

system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH _1.680_0.36N_0.22; these cells contain 6% ash. What is the maximum possible biomass yield from methanol?

#Question id: 14318

#Section 5: Bioprocess Engineering and Process Biotechnology

system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH _1.680_0.36N_0.22; these cells contain 6% ash. If the actual yield of biomass from methanol is 42% the thermodynamic maximum, what is the oxygen demand?

#Question id: 14319

#Section 5: Bioprocess Engineering and Process Biotechnology

Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g^-1 for yeast and 0.05 g g^-I for Z. mobilis. In both cases the nitrogen source is NH₃. Both cell compositions are represented by the formula CH_1.80_0.5N_0.2. What is the yield of ethanol from glucose by Saccharomyces cerevisiae yeast?