At what time between 6 to 7 o’ clock minute and hour hand will opposite ?

TLS Online TPP Program

#Question id: 17202

#Section 1: Engineering Mathematics

The probability that at least one of the events Q and R occur is 0.6. If Q and R have probability of occurring together as 0.2, then P(Q) + P(R) is..........

TLS Online TPP Program

#Question id: 15698

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following does not describe global alignment algorithm?

TLS Online TPP Program

#Question id: 569

#Section 2: General Biology

A small molecule that decreases the activity of an enzyme by binding to a site other than the catalytic site is termed a(n):

TLS Online TPP Program

#Question id: 13091

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

TLS Online TPP Program

#Question id: 3664

#Section 3: Genetics, Cellular and Molecular Biology

 If a mutation is complemented by a clone of a different gene whose product is being overproduced, this is called: