The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:

C₆H₁₂O₆ + aNH₃ → 0.59 CH_1.64N_0.16O_0.52 (biomass) + 0.43 C₃H₈O₃ + 1.54CO₂ + 1.3C₂H₅OH + 0.036H₂O 

and determine the product yield coefficient Y etoh/s and determine coefficient a.

#Question id: 13071

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13072

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13073

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13075

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.