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TLS Online TPP Program

#Question id: 19964

Respiratory Quotient is given by 

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. moles of CO2 produced / moles of O2 consumed 
  2. moles of O2 consumed / moles of CO2 produced 
  3. moles of biomass produced / moles of O2 consumed 
  4. moles of biomass produced/ moles of CO2 produced
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TLS Online TPP Program

#Question id: 14290

#Section 5: Bioprocess Engineering and Process Biotechnology

Acetobacter aceti converts alcohol to acetic acid according to the schiometric relation

In a vigorously agitated and aerated reactor containing 20 g/l ethanol, the organism produces 16g/l acetic acid and 2g/l was the residual ethanol concentration what are the theoretical and observed yields of acetic acid expressed in g/g ethanol
TLS Online TPP Program

#Question id: 14291

#Section 5: Bioprocess Engineering and Process Biotechnology

In a batch sterilization process if  overall,  heating &  cooling are 32.2, 9.8 & 10.1 respectively, THEN the holding time at 121^o-C of the process is (given that the specific death rate (k) of microorganisms at 121^o-C is 2.54 min-1 ):
TLS Online TPP Program

#Question id: 14292

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider the scale up of a fermentation from a 10L to 10000L while maintaining geometric similarity. Agitation speed was maintained at 500 rpm in the 10L fermenter. If scale up is done based on constant tip speed, then the agitation speed in the larger reactor should be 
rpm. 
TLS Online TPP Program

#Question id: 14293

#Section 5: Bioprocess Engineering and Process Biotechnology

Scale up of a fermenter is done based on constant impeller tip speed. If the diameter of the impeller is increased by 10-fold, the agitator speed will: 
TLS Online TPP Program

#Question id: 14294

#Section 5: Bioprocess Engineering and Process Biotechnology

What will be the generation time of a culture with a specific growth rate constant of 0.01 min^-1? 
TLS Online TPP Program

#Question id: 14295

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas methylotrophus is used to produce single-cell protein from methanol in a 1000 m3 pressure-cycle airlift fermenter. The biomass yield from substrate is 0.41 g g- 1, KS is 0.7 mg l- 1, and the maximum specific growth rate is 0.44 h- 1. The medium contains 4% (w/v) methanol. A substrate conversion of 98% is desirable. The reactor may be operated either in batch or continuous mode. If operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between batches is 20 h. If operated continuously, a downtime of 25 d is expected per year. Neglecting maintenance requirements, compare the annual biomass production from batch and continuous reactors?

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