TLS Online TPP Program

#Question id: 27177


A strain of Azotobacter vinelandii is cultured in a 15-m³ stirred fermenter for alginate produc- tion. Under current operating conditions, kLa is 0.17 s-1. The solubility of oxygen in the broth is approximately 8 × 10-3 kg m-3.
(b) The bacteria suffer growth inhibition after copper sulphate is accidentally added to the fermentation broth just after the start of the culture. This causes a reduction in the oxygen uptake rate to 3 mmol g-1 h-1. What maximum cell concentration can now be supported by oxygen transfer in the fermenter?.....g/l

#Section 5: Bioprocess Engineering and Process Biotechnology
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TLS Online TPP Program

#Question id: 3917

#Section 3: Genetics, Cellular and Molecular Biology

For an RNA hairpin loop to form, the transcribed region of the DNA sequence must __.

TLS Online TPP Program

#Question id: 13108

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 Why is it that some people show three bands?

TLS Online TPP Program

#Question id: 3809

#Section 3: Genetics, Cellular and Molecular Biology

MMR is a highly conserved biological pathway. Several human MMR proteins have been identified based on their homology to E. coli MMR proteins. Match the following

Human

Function

1. MSH2-MSH6

A. DNA mismatch/damage recognition

2. MLH-PMS

B. Molecular matchmaker

3. MutH

C. Strand discrimination

D. Absent in Human


TLS Online TPP Program

#Question id: 360

#Section 4: Fundamentals of Biological Engineering

Which of the following is considered to be a strong base (alkali)?

TLS Online TPP Program

#Question id: 14164

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                  

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g-l?