A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.

(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:

TLS Online TPP Program

#Question id: 3013

#Section 2: General Biology

Bacteria survive adverse conditions by forming

TLS Online TPP Program

#Question id: 13087

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

TLS Online TPP Program

#Question id: 12737

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Name the technique which is used to detect abnormalities at an early stage.

TLS Online TPP Program

#Question id: 14134

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PAM stands for ___

TLS Online TPP Program

#Question id: 16841

#General Aptitude

Which of these images is the top view of the given object?