10 kg of a pure enzyme (Mr 30 000) catalyses the conversion of 1.5 g of a substrate (Mr 44) to products in 5 minutes. What will be the turnover number in (sec^-1)  ....10^5

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#Question id: 14280

#Section 5: Bioprocess Engineering and Process Biotechnology

In microbial cultivation, the expression for product synthesis

TLS Online TPP Program

#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 14282

#Section 5: Bioprocess Engineering and Process Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Section 5: Bioprocess Engineering and Process Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14284

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is 

TLS Online TPP Program

#Question id: 14285

#Section 5: Bioprocess Engineering and Process Biotechnology

Match the transport process with appropriate time constants given below 

Transport process                       time constants

1. Oxygen transfer                     p. V/Q

2. Heat transfer                      q. 4V(1.5ND3)

3. Flow                                     r. VpCp/UA

4. Mixing                              s. 1/KLa