TLS Online TPP Program

#Question id: 27909


A 20-1 stirred fermenter containing a Bacillus thuringiensis culture at 30°C is used for production of microbial insecticide, ka is determined using the dynamic method. Air flow is shut off for a few minutes and the dissolved-oxygen level drops; the air supply is then re-connected. When steady state is established, the dissolved-oxygen tension is 78% air saturation. The following results are obtained. Estimate ka.

#Section 5: Bioprocess Engineering and Process Biotechnology
  1. 0.080 s-1
  2. 0.083 s-1
  3. 0.085 s-1
  4. 0.081 s-1