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TLS Online TPP Program

#Question id: 18808

What would happen if serum is omitted from the ELISA, but all other steps remain same?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Anti-human Ig-conjugate would not bind and be washed away
  2. The OD values would be nearly the same as the assay control
  3. Both (a) and (b)
  4. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
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TLS Online TPP Program

#Question id: 14162

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous process is set up for treatment of wastewater. Each day, 105 kg cellulose and 103 kg bacteria enter in the feed stream, while 104 kg cellulose and 1.5 x 104 kg bacteria leave in the effluent. The rate of cellulose digestion by the bacteria is 7 x 104 kg d-1. The rate of bacterial growth is 2 x 104 kg d-1 ; the rate of cell death by lysis is 5 x 102 kg d-1. Write balances for cellulose and bacteria in the system.
TLS Online TPP Program

#Question id: 3957

#Section 3: Genetics, Cellular and Molecular Biology

In mammalian cells, the DNA of the centromere is characteristic of:
TLS Online TPP Program

#Question id: 13056

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
 Which of the following is true for traditional and real time PCR?
TLS Online TPP Program

#Question id: 3595

#Section 3: Genetics, Cellular and Molecular Biology

Ranch mink with the dark gray coat color known as Aleutian is homozygous recessive aa. Genotypes AA and Aa have the standard deep brown color. A mating of Aa  X Aa produces  four  pups. What is the probability of a perfect 3 : 1 distribution of standard to Aleutian?
TLS Online TPP Program

#Question id: 15863

#Section 6: Plant, Animal and Microbial Biotechnology

what is protoplast?