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TLS Online TPP Program

#Question id: 5301

Which of the following processes is an early step in the whole-genome shotgun approach to sequencing?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

  1. break genomic DNA at random sites

  2. map the position of cloned DNA fragments

  3. randomly select DNA primers and hybridize these to random positions of chromosomes in preparation for sequencing

  4. break genomic DNA at specific sites

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TLS Online TPP Program

#Question id: 14172

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:
In a batch reactor of volume 105 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficients YX/S (biomass/glucose)?
TLS Online TPP Program

#Question id: 14173

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:
In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry. Determine the yield coefficient YX/O2 (biomass/oxygen)?
TLS Online TPP Program

#Question id: 14174

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:


In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry, Determine the total amount of oxygen required?

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TLS Online TPP Program

#Question id: 14175

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the biomass yield coefficient YX/S? 
TLS Online TPP Program

#Question id: 14176

#Section 5: Bioprocess Engineering and Process Biotechnology

 The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficient YEtOH/S 
TLS Online TPP Program

#Question id: 14177

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficients YCO2/S