What is the correct order of steps in the CRISPR process?

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#Question id: 14403

#Section 5: Bioprocess Engineering and Process Biotechnology

Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

TLS Online TPP Program

#Question id: 14404

#Section 5: Bioprocess Engineering and Process Biotechnology

Ultrafiltration is used to filtrate molecules of size 

TLS Online TPP Program

#Question id: 14405

#Section 5: Bioprocess Engineering and Process Biotechnology

If frequency adopted risk of contamination is 1 in 1000. For unsterile broth which having 10^11 viable organism then calculate the del factor? 

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#Question id: 14406

#Section 2: General Biology

Some members of the genus Proteus, as well as other genera, can lose their cell walls and swell into irregularly shaped cells called as 

TLS Online TPP Program

#Question id: 14407

#Section 2: General Biology

Most gram-negative bacteria are not as susceptible to penicillin as gram-positive bacteria are because

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#Question id: 14408

#Section 2: General Biology

Acid-fast bacteria such as Mycobacterium can be stained with