Statement: “Purpose of establishing the mathematical models Such as PSSM and Profiles, is to allow partial matches with a query sequence so they can be used to detect more distant members of the same sequence family, resulting in an increased sensitivity of database searches.”

TLS Online TPP Program

#Question id: 12938

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

What is the main advantage of using Yeast Artificial Chromosome based vector over other vectors?

1. It has ability to clone very large fragments of DNA.

2. YAC vectors do not contain origin of replication sites 

3. It has insert  range up to 2000 kb.

4. It help in genome mapping and sequencing projects.

TLS Online TPP Program

#Question id: 12939

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the incorrect statement for the production of site-directed mutations.

TLS Online TPP Program

#Question id: 12940

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Choose the correct order for the steps involve in Protein engineering.

1. Protein modelling

2. Protein crystallography

3. Site-directed mutations

4. Comparison with entries in databases of known proteins.

TLS Online TPP Program

#Question id: 12941

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the therapeutic protein which has been used to successfully treat certain types of breast cancer.

TLS Online TPP Program

#Question id: 12942

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name a single - stranded uracil containing vector which is grown in a selectable host deficit of the enzymes.

TLS Online TPP Program

#Question id: 12943

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation