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TLS Online TPP Program

#Question id: 36308

Which of the following is/are correct regarding progressive alignment ?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Progressive alignment depends on the stepwise assembly of multiple alignment and is heuristic in nature.
  2. Needleman–Wunsch global alignment method used.
  3. The scores can either be percent identity or similarity scores based on a particular substitution matrix. Both scores correlate with the evolutionary distances between sequences.
  4. Clustal  is a progressive Multiple alignment method.
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TLS Online TPP Program

#Question id: 13031

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Use of alkaline solution in southern blotting helps in :
TLS Online TPP Program

#Question id: 13032

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to
TLS Online TPP Program

#Question id: 13033

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The sample is then fractionated by electrophoresis through a _______. As all the proteins now have an _______ to mass ratio, they are separated on the basis of their _____.
TLS Online TPP Program

#Question id: 13034

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which the following statements is/are true about the stacking gel?
i. The stacking gel solution contains acrylamide.
ii. It is on the top of the separating gel.
iii. It is used to dilute the protein sample before it enters the main separating gel.
iv. It is into this gel that the wells are formed and the proteins loaded.
TLS Online TPP Program

#Question id: 13035

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.
TLS Online TPP Program

#Question id: 13036

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In isoelectric focusing the protein will move until