Lys & Arg have four ionisations zones

1. 1--------pK1 – charge +2

2. pK1---------pK2 charge +1

3. pK2---------pKr charge 0

4. pKr---------14 charge -1

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#XL - Q Biochemistry

A branched oligosaccharide containing 

3 glucose (Glc)

9 mannose (Man)

2 N-acetylglucosamine (GlcNAc)

Glc3Man9(GlcNAc)2

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#XL - Q Biochemistry

Only asparagine residues in the tripeptide sequences Asn-X-Ser and Asn-X-Thr (where X is any amino acid except proline) are substrates for oligosaccharyl transferase.

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#Id: 7954

#XL - Q Biochemistry

The precursor oligosaccharide is transferred from a

dolichol lipid anchor to the asparagine as an intact unit in a reaction catalyzed by a transmembrane oligosaccharyl transferase enzyme complex.

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#Id: 7953

#XL - Q Biochemistry

Tunicamycin

blocks the first enzyme in this pathway, inhibits the synthesis of all N-linked oligosaccharides in cells.

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#Id: 7952

#XL - Q Biochemistry

Two N- acetylglucosamine (GlcNAc) and five mannose residues are added one at a time to a dolichol phosphate on the cytosolic face of the ER membrane

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#Id: 7951

#XL - Q Biochemistry

First sugar residue is attached to dolichol by a high-energy pyrophosphate linkage & the other sugars are added by glycosidic bonds