Two major classes of actin-nucleating proteins:

1. Formins nucleate the assembly of unbranched filaments 

2. Arp2/3 complex nucleates the assembly of branched actin networks

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#XL - Q Biochemistry

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.

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#XL - Q Biochemistry

The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

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#XL - Q Biochemistry

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond. 

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#XL - Q Biochemistry

The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.

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#XL - Q Biochemistry

The irreversible inhibitors cannot be removed by simple physical techniques such as dialysis, is to reduce the amount of enzyme available for reaction.

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#XL - Q Biochemistry