In unstimulated cells, 

Raf is phosphorylated by several cytosolic kinases on two sites:

1. Serine/threonine kinase domain 

2. N-terminal autoinhibitory domain

TLS Online TPP Program

#Id: 9714

#XL - T Zoology

Ste11 analogous to Raf and other mammalian MEK kinase (MEKK) proteins.

TLS Online TPP Program

#Id: 9715

#XL - T Zoology

Activated Ste11 then phosphorylates Ste7, a dual-specificity MEK.

TLS Online TPP Program

#Id: 9716

#XL - T Zoology

Ste7, phosphorylates and activates Fus3,  a serine/threonine kinase

TLS Online TPP Program

#Id: 9717

#XL - T Zoology

Fus3 equivalent to MAP kinase

TLS Online TPP Program

#Id: 9718

#XL - T Zoology

Fus3 translocates into the nucleus & phosphorylates two proteins, Dig1 and Dig2.

TLS Online TPP Program

#Id: 9719

#XL - T Zoology

Phosphorylated Dig1 and Dig2, relieves the inhibition of the Ste12 transcription factor.