DDT was once considered a ʺsilver bulletʺ that would permanently eradicate insect pests. Today, instead, DDT is largely useless against many insects. Which of these would have been required for this pest eradication effort to be successful in the long run?

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#Question id: 13068

#XL - S Microbiology

Coating antibody; which actively traps antigen

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#Question id: 13069

#XL - S Microbiology

A negative agglutination test may NOT indicate the

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#Question id: 13071

#XL - S Microbiology

Modifications of the agglutination reaction involve the use of ________particles, which allow the reaction to be observed more easily in the liquid phase

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#Question id: 13073

#XL - S Microbiology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;

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#Question id: 13075

#XL - S Microbiology

Radioisotopes must be used in RFLP and RAPD markers

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#Question id: 13076

#XL - Q Biochemistry

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.