TLS Online TPP Program

#Question id: 12917


Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

#XL - S Microbiology
  1. 1-2-3-4
  2. 2-1-4-3
  3. 4-3-2-1
  4. 3-4-1-2