Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 148

#XL - T Zoology

The pKa of a certain weak acid is 4.0. Calculate the ratio of proton acceptor to proton donor at pH 7.0.

TLS Online TPP Program

#Question id: 149

#XL - T Zoology

The pKaʹs of the side chain group and the α-carboxyl group of glutamate are 4.1 and 2.1, respectively. Which statement accounts for this difference?

TLS Online TPP Program

#Question id: 150

#XL - T Zoology

How do the pKa values of an ionizable side chain compare when the amino acid is free versus when it is in a polypeptide chain?

TLS Online TPP Program

#Question id: 151

#XL - T Zoology

The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pHCOOH = 2.0; pHNH2= 10.6), and the mixture is placed in an electric field at pH 6.15. Which statement is true?

TLS Online TPP Program

#Question id: 152

#XL - T Zoology

According to the Henderson-Hasselbalch equation, when the concentrations of proton acceptor and proton donor are the same, then

TLS Online TPP Program

#Question id: 153

#XL - T Zoology

For the amino acid lysine, the Henderson-Hasselbalch equation can be applied to ________ ionization group(s)