TLS Online TPP Program

#Question id: 13031


Use of alkaline solution in southern blotting helps in :

#XL - S Microbiology
  1. Transfer of the blot to nitrocellulose membrane
  2. Depurination of DNA fragments and hence breaking them into smaller fragments
  3. Denaturation of the DNA fragments helping in probe hybridization
  4. Binding of negatively charged DNA to the membrane
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TLS Online TPP Program

#Question id: 446

#XL - S Microbiology

Which statement is false about ATP?

TLS Online TPP Program

#Question id: 447

#XL - S Microbiology

In glycolysis, fructose 1,6-bisphosphate is converted to two products with a standard free-energy change (DG'°) of 23.8 kJ/mol.  Under what conditions encountered in a normal cell will the free-energy change (DG) be negative, enabling the reaction to proceed spontaneously to the right?

TLS Online TPP Program

#Question id: 448

#XL - S Microbiology

During glycolysis, glucose 1-phosphate is converted to fructose 6-phosphate in two successive reactions: Glucose 1-phosphate -> glucose 6-phosphate DG'° = –7.1 kJ/mol Glucose 6-phosphate -> fructose 6-phosphate DG'° = +1.7 kJ/mol DG'° for the overall reaction is:

TLS Online TPP Program

#Question id: 449

#XL - S Microbiology

The standard free-energy changes for the reactions below are given. Phosphocreatine -> creatine + Pi DG'° = –43.0 kJ/mol ATP ->  ADP + Pi DG'° = –30.5 kJ/mol What is the overall DG'° for the following reaction? Phosphocreatine + ADP -> creatine + ATP

TLS Online TPP Program

#Question id: 450

#XL - S Microbiology

The DG'° values for the two reactions shown below are given.

Oxaloacetate + acetyl-CoA + H2O -> citrate + CoASH DG'° = –32.2 kJ/mol

Oxaloacetate + acetate    ->   citrate DG'° = –1.9 kJ/mol

What is the DG'° for the hydrolysis of acetyl-CoA?

Acetyl-CoA + H2O -> acetate + CoASH + H+

TLS Online TPP Program

#Question id: 459

#XL - S Microbiology

Calculate △G°ʹ for the reaction shown if the mass action ratio, Q, is 5.3 × 102 M-1 and △G is 46 kJ/mol at a temperature of 298 K. (R = 8.315 J/K-mol)

ADP + Pi ⇌ ATP