TLS Online TPP Program

#Question id: 5281


In the mating Hfr Strs met+ his+ leu+ trp+ Ala+   X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:

Interrupted time

Composition of media

Growth

His

Leu

Ala

Trp

Met

2   min

+

-

+

+

+

Yes

10 min

+

-

-

+

+

Yes

12 min

+

-

-

+

-

No

15 min

+

-

-

-

+

Yes

20 min

-

-

-

-

+

Yes

What is the order of transfer of the genes?

#XL - S Microbiology
  1. Leu- Ala- His- Met - Trp 

  2. Leu- Trp- His- Met-Ala       

  3. Ala- Trp- His- Met- Leu   

  4. Leu- Ala- Trp- His- Met