In which way is the organizer region of the sea urchin similar to that in frogs?

#Question id: 5290

#XL - S Microbiology

The following table shows mapping data from three interrupted mating experiments using three different Hfr strains and an F- strain: The order of genes is

#Question id: 2655

#XL - T Zoology

Lac Operon will be turned on when

#Question id: 3933

#XL - Q Biochemistry

A gene contains two 100 bp introns and three 50 bp exons. About how long is the mature RNA transcribed from this gene?

#Question id: 19967

#XL - P Chemistry

#Question id: 13076

#XL - Q Biochemistry

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.