TLS Online TPP Program

#Question id: 10290


In glycolysis, perform succesfully ten step reaction, the conversion of 1 molecules of glucose 6-phosphate to 2 molecules of Pyruvate. What is the net gain of overall reaction?

#XL - Q Biochemistry
  1. 1 mol of NAD+ and 2 mol of ATP
  2. 2 mol of NADH and 2 mol of ATP
  3. 2 mol of NADH and 4 mol of ATP
  4. 2 mol of NAD+ and 4 mol of ATP
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TLS Online TPP Program

#Question id: 5279

#XL - S Microbiology

In a transduction experiment, phage P1 is grown on a bacterial host of genotype A+ B+ C+ and the resulting lysate is used to infect a recipient strain of genotype A– B– C–. The genes are in an order such that B is in the middle, and the distance between A and B is greater than the distance between B and C. Based on this information, which of the following least genotype?

TLS Online TPP Program

#Question id: 5281

#XL - S Microbiology

In the mating Hfr Strs met+ his+ leu+ trp+ Ala+   X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:

Interrupted time

Composition of media

Growth

His

Leu

Ala

Trp

Met

2   min

+

-

+

+

+

Yes

10 min

+

-

-

+

+

Yes

12 min

+

-

-

+

-

No

15 min

+

-

-

-

+

Yes

20 min

-

-

-

-

+

Yes

What is the order of transfer of the genes?

TLS Online TPP Program

#Question id: 5285

#XL - S Microbiology

A transformation experiment is carried out using donor DNA that is A+B+C+ and a recipient that is A-B- C-. A+ transformants are selected and tested further. Of these, 64% are B+ and none are C+. Also, B+ are selected, and 8% are also C+. What is the gene order

TLS Online TPP Program

#Question id: 5287

#XL - S Microbiology

Interrupted matting of different Hfr strain with F- E.coli cell resulting generation of following strain  Order of gene is

TLS Online TPP Program

#Question id: 5290

#XL - S Microbiology

The following table shows mapping data from three interrupted mating experiments using three different Hfr strains and an F- strain: The order of genes is

TLS Online TPP Program

#Question id: 5295

#XL - S Microbiology

A cross is made between an Hfr that is met+ thi+ pur+ and an F-that is met- thi- pur-. Interrupted mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements.  These recombinants are tested for the presence of the thi+ and pur+ alleles. The following numbers of individuals are found for each genotype:

A) Gene Order

I

Thi Pur  Met

II

Pur  Met  Thi

III

Pur   Thi  Met

B) Distance between Pur and Thi

IV

15.4 map unit

V

1.8 map unit

What is the following correct combination?