TLS Online TPP Program

#Question id: 563


Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that:

#XL - Q Biochemistry
  1. glucose has more —OH groups per molecule than does water.

  2. the larger glucose binds better to the enzyme; it induces a conformational change in hexokinase that brings active-site amino acids into position for catalysis.

  3. the —OH group of water is attached to an inhibitory H atom, while the glucose —OH group is attached to C.

  4. water and the second substrate, ATP, compete for the active site resulting in a competitive inhibition of the enzyme.