Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 7286

#XL - R Botany

Which of the floral whorls is affected in apetala 3/pistillata (ap3/pi) mutants?

#Question id: 7288

#XL - R Botany

Following statements are regarding to the expression of genes in floral quartate model in Arabidopsis thaliana.

A. Sepals- AP1(heterodimer) + SEP(homodimer)

B. Petals-AP1(monomer)+PI(monomer)+AP3(monomer)+SEP(monomer)

C. Stamens- AP3(monomer)+PI(monomer)+AG(monomer)+SEP(monomer)

D. Carpels- AG(homodimer)+SEP(homodimer)

Which of the following genetic combinations are incorrect?

#Question id: 7289

#XL - R Botany

The following scheme shows the flowering status of a plant species and the photoperiodism. Which of the following conclusions is most appropriate?

#Question id: 7290

#XL - R Botany

Following figure regarding to regulatory network in the Arabidopsis shoot meristem stem-cell niche.

A. The transcription factor WUS is synthesized in the cell A and moves via plasmodesmata into cell B, where one of its functions is to induce expression of the CLV3.

B. The transcription factor CLV3 is synthesized in the cell A and moves via plasmodesmata into cell B, where one of its functions is to induce expression of the WUS.

C. Secreted CLV3 protein from cell B binds to the receptor protein kinase on the surface of cell A; there, it activates a signal that represses WUS transcription.

D. Secreted WUS protein from cell B binds to the receptor protein kinase on the surface of cell A; there, it activates a signal that represses CLV3 transcription.

Which of the following combination is correct?

#Question id: 7291

#XL - R Botany

Five different genes are known to specify floral organ identity in Arabidopsis: APETALA1 (AP1), APETALA2 (AP2), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG).

Which one of the following statement is incorrect?

#Question id: 7292

#XL - R Botany

How is plant cell cytokinesis different from animal cell cytokinesis?