Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 4734

#XL - R Botany

If a homozygous plant with red peppers is crossed with a homozygous plant with green peppers, all the F1 plants have red peppers When the F1 are crossed with one another then produced following progeny Which of following interaction involved in gene to gene of peppers plant?

#Question id: 4742

#XL - R Botany

In birds, sex is determined by a ZW chromosome scheme. Males are ZZ and females are ZW. A recessive lethal allele that causes death of the embryo is sometimes present on the Z chromosome in pigeons. What would be the sex ratio in the offspring of a cross between a male that is heterozygous for the lethal allele and a normal female?

#Question id: 4743

#XL - R Botany

Ectrodactyly is an autosomal dominant trait that causes missing middle fingers (lobster claw malformation). A grandfather and grandson both have ectrodactyly, but the intervening father has normal hands by x-ray. Which of the following terms applies to this family?

#Question id: 4744

#XL - R Botany

Assume that long ear lobes in humans are an autosomal dominant trait that exhibits 50% penetrance. A person who is heterozygous for long ear lobes mates with a person who is homozygous for normal ear lobes. What is the probability that their first child will have long ear lobes.

#Question id: 4747

#XL - R Botany

How does incomplete dominance differ from incomplete penetrance?

#Question id: 4749

#XL - R Botany

A normal woman, whose father was Hemophilic and color blind is married to a colorblind, nonhemophilic man. What will be probability of colorblind, hemophilic daughter?