Which of these is able to cross the inner mitochondrial membrane?

TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.

1. YFP

2. RFP

3. GFP

4. CFP

Which option below contains correct combination of flurorescent molecules that can give FRET?

TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as

1.  Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?

TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?