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TLS Online TPP Program

#Question id: 11406

Which of the following is true for a critically endangered species?

#Unit 10. Ecological Principles
  1. Reduction in population size is greater than 90%; estimated extinction risk at least 80% in 10 years; area of occupancy less than 10 km^2 generations
  2. Reduction in population size is greater than 90%; estimated extinction risk at least 50% in 10 years; area of occupancy less than 100 km^2 generations
  3. Reduction in population size is greater than 90%; estimated extinction risk at least 50% in 10 years; extent of occurrence less than 10 km^2 generations
  4. Reduction in population size is greater than 90%; estimated extinction risk at least 50% in 10 years; area of occupancy less than 10 km^2 g 
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TLS Online TPP Program

#Question id: 23181

#Unit 13. Methods in Biology

__________ type of biosensors exist to measure the concentration and location of Ca2+ and the activation state of GTPase switch proteins.
TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than 
TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.
1. YFP
2. RFP
3. GFP
4. CFP
Which option below contains correct combination of flurorescent molecules that can give FRET?
TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as
1.  Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?