#Question id: 4116
#Unit 3. Fundamental Processes
Proteins that regulate splicing bind to specific sites called exonic (or intronic) splicing enhancers (ESE or ISE) or silencers (ESS and ISS). The former enhances, and the latter repress, splicing at nearby splice sites. Examples of splicing activator and repressors are as
#Question id: 4115
#Unit 3. Fundamental Processes
The possible type of splicing outcome of given gene construct will be
#Question id: 4114
#Unit 3. Fundamental Processes
Splice-site recognition is prone to two kinds of errors. First, splice sites can be skipped, with components bound at, for example, a given 5’ splice site pairing with those at a 3’ site beyond the correct one. Ways in which the accuracy of splice-site selection can be enhanced are as
A. The factors that recognize that site are transferred from the polymerase carboxy-terminal “tail” onto the RNA. Once in place, the 5’ splice site components are poised to interact with those other factors that bind to the next 3’ splice site to be synthesized.
B. SR (serine–arginine-rich) proteins bind to sequences called exonic splicing enhancers (ESEs) within the exons. SR proteins bound to these sites recruit the splicing machinery to the nearby splice sites to ensure correct splicing
C. By recruiting splicing factors to each side of a given exon, this process encourages the so-called “exon definition, contribute to correct splicing
D. Alternative splicing is intentional missplicing mechanism
Which of the following is INCORRECT?
#Question id: 4113
#Unit 3. Fundamental Processes
Match the transcriptional machinery with their respective RNA Polymerase
#Question id: 4112
#Unit 3. Fundamental Processes
Which of the following is the structure of the nucleotide at the branch site of a spliced intron?
#Question id: 4111
#Unit 3. Fundamental Processes
Which of the following is NOT characteristic of a eukaryotic enhancer element?