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TLS Online TPP Program

#Question id: 4976

Which of these is the most likely explanation for the existence of dicynodont fossils on modern-day Antarctica?

#Unit 11. Evolution and Behavior

  1. They arrived there aboard ʺraftsʺ of vegetation, and quickly adapted to the bitterly cold climate.

  2. Earthʹs polar regions were once so warm (especially immediately after the ʺsnowball Earth periodʺ) that reptiles and mammal-like reptiles flourished there.

  3. The landmass that is now the Antarctic continent was formerly located at a more-northerly position, and was also united to other landmasses.

  4. Dicynodonts originated on the island continent of Antarctica and went extinct as the continent migrated to its current position at the South Pole.

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TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.
1. YFP
2. RFP
3. GFP
4. CFP
Which option below contains correct combination of flurorescent molecules that can give FRET?
TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as
1.  Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

 Column I Column II 
 1. Texas a. far red
 2. Fluorescein b. red
 3. Rhodamine c. blue
 4. Cy5 d. green
Which of the following option comprises all correct matches?