#Question id: 4892
#Unit 8. Inheritance Biology
The RFLP pattern for this locus, obtained by hybridization using a probe internal sites, from family is shown below: Based on the above profile, what is the nature of the genetic disorder?
#Question id: 4910
#Unit 8. Inheritance Biology
For a trait known to be caused by a mitochondrial gene variant, there may be an occasional female that can transmit different phenotypes to her progeny on an apparently random basis. What situation might you suspect?
#Question id: 20987
#Unit 12. Applied Biology
#Question id: 24121
#General Aptitude
#Question id: 4104
#Unit 3. Fundamental Processes
Consider following statements for catalytic mechanism of RNA polymerase
A. RNA synthesis by RNA polymerase. The reaction involves two Mg2+ ions, coordinated to the phosphate groups of the incoming nucleoside triphosphates (NTPs)
B. Three Asp residues, which are highly conserved in the RNA polymerases of all species. One Mg2+ ion facilitates attack by the 39-hydroxyl group on the phosphate of the NTP; the other Mg2+ ion facilitates displacement of the pyrophosphate, and both metal ions stabilize the pentacovalent transition state.
C. About 17 bp of DNA are unwound at any given time. RNA polymerase and the transcription bubble move from left to right along the DNA as shown, facilitating RNA synthesis. Movement of an RNA polymerase along DNA tends to create negative supercoils (overwound DNA) ahead of the transcription bubble and positive supercoils (underwound DNA) behind it.
D. The RNA polymerase is in close contact with the DNA ahead of the transcription bubble as well as with the separated DNA strands and the RNA within and immediately behind the bubble. A channel in the protein funnels new NTPs to the polymerase active site. The polymerase footprint encompasses about 35 bp of DNA during elongation.