#Question id: 5288
#Unit 8. Inheritance Biology
Donor strain of bacteria with His+ Ara+ Met+ Leu+ Cys+ Lys+ is infected with phages to map the donor chromosome with generalized transduction .The Phage lysate from bacterial cell is collected and use to infect second strain of bacteria that are His- Ara- Met- Leu- Cys- Lys- . Different transducing phages are selected on using different minimal media and their presence of growth (+) and absence of growth (-) given in table
|
Media |
Media supplement 0 absent /+ present |
||||||
|
1 |
His |
Ara |
Met |
Leu |
Cys |
Lys |
Growth |
|
2 |
+ |
+ |
0 |
+ |
+ |
0 |
+ |
|
3 |
0 |
+ |
+ |
+ |
+ |
0 |
+ |
|
4 |
+ |
+ |
0 |
+ |
0 |
+ |
- |
|
5 |
0 |
+ |
+ |
0 |
+ |
+ |
+ |
|
6 |
+ |
0 |
+ |
0 |
+ |
+ |
+ |
|
7 |
+ |
0 |
+ |
+ |
0 |
+ |
+ |
Which of the following is correct order of gene?
#Question id: 5287
#Unit 8. Inheritance Biology
Interrupted matting of different Hfr strain with F- E.coli cell resulting generation of following strain Order of gene is
#Question id: 5286
#Unit 8. Inheritance Biology
An Hfr strain with genotype A B C D (each gene for amino acid synthesis )and that transfers the A gene very late was mated with recipient strain with genotype a b c d. After mating for several hours, the mixture was diluted and plated on minimal media with four different supplements having various number of colony
Media supplement with amino acid
Colony number
A+C 500
C+D 50
B 10
A 250
What is order of gene
#Question id: 5285
#Unit 8. Inheritance Biology
A transformation experiment is carried out using donor DNA that is A+B+C+ and a recipient that is A-B- C-. A+ transformants are selected and tested further. Of these, 64% are B+ and none are C+. Also, B+ are selected, and 8% are also C+. What is the gene order
#Question id: 5284
#Unit 8. Inheritance Biology
DNA from a strain of Bacillus subtilis with genotype a+b+ c+d+ e+ is used to transform a strain with genotype a - b- c- d- e-. Pairs of genes are checked for co-transformation and the following results are obtained:
Pair of co-transform genes as follow
D-E, E-C, C-B, A-D
On the basis of these results, what is the order of the genes on the bacterial chromosome?
#Question id: 5283
#Unit 8. Inheritance Biology
A generalized transducing phage is used to transduce an a- b- c- d- e- recipient strain of E. coli with an a+ b+c+d+ e+ donor. The recipient culture is plated on various media with the results shown in the table below What can you conclude about the linkage and order of the genes?