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#Question id: 882


Choose correct statements about thermophilic proteins

I. The proteins found in thermophilic species are much more stable than their mesophilic counterparts.

II. All thermostable proteins have such a high incidence of salt bridges

III. the most important of which are an increased size of the protein’s hydrophobic core, an increased size of the interface between its domains and/or subunits, and a more tightly packed core as evidenced by a reduced surface-to-volume ratio.

IV. Several of these thermostable enzymes have a superabundance of salt bridges on their surfaces.

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. I, II and IV

  2. II, III and IV

  3. III and IV

  4. I, III and IV

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TLS Online TPP Program

#Question id: 5282

#Unit 8. Inheritance Biology

Conjugation allowed between Hfr with genotype A B C D E and F- with genotype a b c d e. At regular intervals, a sample of cells was removed and agitated

vigorously in a kitchen blender to halt all conjugation and DNA transfer. After interrupted conjugation, ex-conjugate were selected on minimal media at regular interval and find different number of ex-conjugate?

abCde   - 10, abCdE    -    30 ,  aBCdE  -     40 , ABCdE  -     20 , ABCDE   -   50

What is gene order of those genes?

TLS Online TPP Program

#Question id: 5281

#Unit 8. Inheritance Biology

In the mating Hfr Strs met+ his+ leu+ trp+ Ala+   X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:

Interrupted time

Composition of media

Growth

His

Leu

Ala

Trp

Met

2   min

+

-

+

+

+

Yes

10 min

+

-

-

+

+

Yes

12 min

+

-

-

+

-

No

15 min

+

-

-

-

+

Yes

20 min

-

-

-

-

+

Yes

What is the order of transfer of the genes?

TLS Online TPP Program

#Question id: 5280

#Unit 8. Inheritance Biology

In the mating Hfr met- his+ leu+ trp+ × F- met+ his- leu- trp-.  The met marker is known to be transferred very late. After a short time, the mating cells are interrupted and the cell suspensions plated on four different growth media. The amino acids in the growth media and the number of colonies observed on each are as follows:

histidine + tryptophan  250 colonies

histidine + leucine         50 colonies 

leucine + tryptophan    500 colonies

histidine                       10 colonies

What is the order of transfer of the genes

TLS Online TPP Program

#Question id: 5279

#Unit 8. Inheritance Biology

In a transduction experiment, phage P1 is grown on a bacterial host of genotype A+ B+ C+ and the resulting lysate is used to infect a recipient strain of genotype A– B– C–. The genes are in an order such that B is in the middle, and the distance between A and B is greater than the distance between B and C. Based on this information, which of the following least genotype?

TLS Online TPP Program

#Question id: 5278

#Unit 8. Inheritance Biology

What is the co-transduction frequency for the A and B genes, from the following dataset?(Assume that there has been selection for the A+ form of the A gene). Genotype                                Number

 A+B+C+                                   10

 A+B+C-                                    30

A+B-C+                                     20

 A+B-C-                                     40

TLS Online TPP Program

#Question id: 5277

#Unit 8. Inheritance Biology

Deduce the genotype of bacteria strain was shown either growth presents or not in different media?

Media supplement

Minimal

Minimal + Arginine

Minimal + Methionine

Minimal + Arginine+ Methionine

Growth

No

Yes

     No

Yes