Correct approaches to protein structure prediction-:

i. Homology modeling	a) Methods is the Rosetta program, formulated by David Baker. To satisfy the program’s computational needs, a volunteer network of ∼100,000 computers, known as Rosetta@home, provides the 500,000 or so hours of processing time required to generate a structure.
ii. Structural genomics	b) which seeks to determine the X-ray structures of all representative domains, is aimed at expanding this predictive technique. The identification of structural homology is likely to provide clues as to a protein’s function even with imperfect structure prediction.
iii. Threading	c) Is a computational technique that attempts to determine the unknown structure of a protein by ascertaining whether it is consistent with a known protein structure. It does so by placing the unknown protein’s residues along the backbone of a known protein structure and then determining whether the amino acid side chains of the unknown protein are stable in that arrangement
iv. Ab initio	d) Aligns the sequence of interest with the sequence of a homologous protein or domain of known structure—compensating for amino acid substitutions, insertions, and deletions—through modeling and energy minimization calculations.

#Question id: 623

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of these statements about enzyme-catalyzed reactions is false?

#Question id: 622

#Unit 1. Molecules and their Interaction Relevant to Biology

An enzyme-catalyzed reaction was carried out with the substrate concentration initially a thousand times greater than the Km for that substrate.  After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 micromol.  If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how long would it take for the same amount (12 micromol) of product to be formed?

#Question id: 621

#Unit 1. Molecules and their Interaction Relevant to Biology

Michaelis and Menten assumed that the overall reaction for an enzyme-catalyzed reaction could be written as Using this reaction, the rate of breakdown of the enzyme-substrate complex can be described by the expression:

#Question id: 620

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following statements about a plot of V0 vs. [S] for an enzyme that follows Michaelis-Menten kinetics is false?

#Question id: 619

#Unit 1. Molecules and their Interaction Relevant to Biology

The benefit of measuring the initial rate of a reaction V0 is that at the beginning of a reaction:

#Question id: 618

#Unit 1. Molecules and their Interaction Relevant to Biology

 In the following diagram of the first step in the reaction catalyzed by the protease chymotrypsin, the process of general base catalysis is illustrated by the number ________, and the process of covalent catalysis is illustrated by the number _________.