The transition from the closed to the open complex involves structural changes in the enzyme and the opening of the DNA double helix to reveal the template and nontemplate strands. This “melting” involves

A. Isomerization reaction and it does not require energy derived from ATP hydrolysis

B. Isomerization is essentially irreversible and, once complete, typically guarantees that transcription will subsequently initiate

C. Positions –11 and -2, with respect to the transcription start site.

TLS Online TPP Program

#Question id: 19875

#Unit 13. Methods in Biology

which type of restriction endonuclease cleaves DNA in the immediate vicinity of their recognition sites, such as Eco Pl, EcoP15 etc.

TLS Online TPP Program

#Question id: 19878

#Unit 13. Methods in Biology

The gene formed by the joining of a DNA from on organism joining to the regulatory sequences of another organism known as

TLS Online TPP Program

#Question id: 19879

#Unit 13. Methods in Biology

Which of the following enzyme is used to cut DNA molecules in rDNA technology

TLS Online TPP Program

#Question id: 19880

#Unit 13. Methods in Biology

Restriction enzymes are also called as

TLS Online TPP Program

#Question id: 19881

#Unit 13. Methods in Biology

The DNA moleclule to which the gene of insert is integrated for cloning is called

TLS Online TPP Program

#Question id: 19882

#Unit 13. Methods in Biology

The vector containing the DNA fragment to be cloned