TLS Online TPP Program

#Question id: 4118


If the 5’ splice site sequence changed from 5’- GUAAGU-3’ to 5’-GUAUGU-3’, predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein– RNA binding assay.

#Unit 3. Fundamental Processes
  1. The mutation will not change binding of U1 and on other hand this will likely enhance binding of the U6 snRNP with the 5’ splice site

  2. The mutation will likely cause an increase in binding of U1 and on other hand this will not change binding of the U6 snRNP with the 5’ splice site

  3. The mutation will likely cause a decrease in binding of U1 and on other hand this will likely enhance binding of the U6 snRNP with the 5’ splice site

  4. The mutation will likely cause a decrease in binding of U1 and on other hand this will not change binding of the U6 snRNP with the 5’ splice site