In a biochemical experiment, you compare the products from splicing reactions carried out in vitro using three different substrates. In each case the substrate is a construct containing a single intron surrounded by two exons, and in all cases the construct is the same overall size. But in one case, the intron is a group I intron, in another a group II intron, and in the third an intron removed by the spliceosome. Each construct is labelled in a manner that allows it to be detected after gel electrophoresis, and each is tested in two reactions—one, conditions that support self-splicing, and two, in the presence of nuclear extract as well. Note that, for simplification, only the final products of the splicing reaction are seen, but before degradation of the introns.

 Which of the following observation is/are correct?

A. In lane A, the presence of nuclear abstract, a band for the lariat and the spliced product and pre-mRNA.

B. Lane B shows Group I Introns and Lane C is for Group II introns and reaction is nonself-splicing

C. Lane C is Group I intron. Splicing takes place in the absence and presence of nuclear abstract, and the reaction is self-splicing.

#Question id: 4882

#Unit 8. Inheritance Biology

In Drosophila melanogaster, cinnabar eye (cn)and vestigial wing (vg) are simple recessive traits.a A male pure line wild type eye ,vestigial wing cross with female pure line cinnabar eye, wild type wing . F1female, heterozygous for both genes, was crossed with a male with cinnabar eyes and vestigial wings. The offspring resulting from this cross are listed in below class

I Wild type

II Cinnabar eye, wild-type wing

III Wild-type eye, vestigial wing

IV Cinnabar eye, vestigial wing

Above class are recombinant progeny

#Question id: 4881

#Unit 8. Inheritance Biology

A panel of cell lines was created from mouse–human somatic-cell fusions. Each line was examined for the presence of human chromosomes and for the production of three enzymes. The following results were obtained On the basis of above results. Which of the following chromosome location of enzyme 1, enzyme 2, and enzyme 3?

#Question id: 4880

#Unit 8. Inheritance Biology

There is following normal gene and their restriction site are given

 Mutant phenotype due to mutation that alters EcoRI site 1 occurs in this piece of DNA. There is following analysis of different band in their family (+ present , - absent) 

 Which of the following is mode of inheritance of trait?

#Question id: 4879

#Unit 8. Inheritance Biology

A wild plant with genes AABBCC was crossed with a triple recessive mutant aabbcc. The F1 male hybrid (AaBbCc) was then back crossed with the triple mutant and the phenotypes recorded are as follows

AabbCc (50), aaBbcc(50), AaBbCc (100), aabbcc (100), AaBbcc (350), aabbCc (350)

Distance between A to B is ----------M----- map unit with ………N…….configuration, distance between B to C is ----O---- map unit with ------P-------configuration?

#Question id: 4719

#Unit 8. Inheritance Biology

When a trait exhibits complete co-dominance rather than complete dominance, a cross between heterozygote results deviation of following;

#Question id: 4723

#Unit 8. Inheritance Biology

The most common cause of the pleiotropic effect of gene is due to