Following statements are regarding to protein kinase A which phosphorylates multiple intracellular target proteins expressed in different cell types.

A. Inactive PKA is a tetramer consisting of two regulatory (R) subunits and two catalytic (C) subunits.

B. Each R subunit contains a pseudo-substrate domain whose sequence resembles that of a peptide substrate and binds to the active site in the catalytic domain but is not phosphorylated; thus the pseudo-substrate domain inhibits the activity of the catalytic subunits.

C. active PKA is turned off by binding of cAMP Each R subunit has two distinct cAMP-binding sites, called CNB-A and CNB-B.

D. Binding of cAMP by an R subunit of PKA occurs in a cooperative fashion; that is, binding of the first cAMP molecule to CNB-B increases the Kd for binding of the second cAMP to CNB-A. Thus small changes in the level of cytosolic cAMP can cause proportionately large changes in the number of dissociated C subunits and, hence, in cellular kinase activity.

Which of the following statements are incorrect?

#Question id: 366

#Unit 1. Molecules and their Interaction Relevant to Biology

What is the pH of a solution with a hydroxyl ion (OH-) concentration of 10-10 M?

#Question id: 365

#Unit 1. Molecules and their Interaction Relevant to Biology

Water is a polar molecule: the oxygen atom with its unshared electrons carries a partial negative charge (δ−) of ___________, and the hydrogen atoms each carry a partial positive charge (δ+) of __________, where e is the charge of the electron.

#Question id: 364

#Unit 1. Molecules and their Interaction Relevant to Biology

 A solution contains 0.0000001 (10-7) moles of hydrogen ions [H+] per liter. Which of the following best describes this solution?

#Question id: 363

#Unit 1. Molecules and their Interaction Relevant to Biology

What is the pH of a mixture of 0.042 M NaH2PO4 and 0.058 M Na2HPO4? If 1.0 mL of 10.0 M NaOH is added to a liter of the buffer prepared, how much will the pH change? (pKa = 6.86)

#Question id: 362

#Unit 1. Molecules and their Interaction Relevant to Biology

A 0.01 M solution of a substance has a pH of 2. What can you conclude about this substance?

#Question id: 361

#Unit 1. Molecules and their Interaction Relevant to Biology

Calculate the percentage of histidine that has its imidazole side chain protonated at pH 7.3. The pKa values for histidine are pK1 = 1.8, pK2 (imidazole) = 6.0, and pK3 = 9.2.