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#Question id: 5670


Embryonic stem cells can be induced in the laboratory to differentiate into muscle, nerve, pancreatic, and other specialized cell types. It has recently become possible to trigger the dedifferentiation of somatic cells back into pluripotent cells (so-called induced pluripotent stem cells or iPS cells) by artificially enhancing the production of a relatively small number of key transcriptional factors known to be required for maintenance of pluripotency. Examples of such transcription factors are OCT4 and NANOG. The production of OCT4 and NANOG (and other key regulatory proteins) is stimulated by a transcription factor known as FOXP1, a member of the Forkhead family of DNA-binding proteins. There are two isoforms of FOXP1.

Which of the following statement regarding to FOXP1 is incorrect?

#Unit 5. Developmental Biology
  1. The protein encoded by the exon 18b–carrying mRNA is called FOXP1-ES: this activates genes (OCT4, NANOG, etc.) that promote dedifferentiation, and thus stimulates iPS cell formation.

  2. In the exon 18a–containing form encodes FOXP1 itself, and this has the opposite effect: it fails to stimulate expression of OCT4 and NANOG and instead actives genes that promote differentiation.

  3. FOXP1 binds to DNA via a domain known as a Winged helix, which recognizes a particular DNA sequence.

  4. The switch from exon 18a to exon 18b substitutes 3 residues in the Winged helix region of the protein including six amino acids that are known to contact DNA.

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TLS Online TPP Program

#Question id: 5280

#Unit 8. Inheritance Biology

In the mating Hfr met- his+ leu+ trp+ × F- met+ his- leu- trp-.  The met marker is known to be transferred very late. After a short time, the mating cells are interrupted and the cell suspensions plated on four different growth media. The amino acids in the growth media and the number of colonies observed on each are as follows:

histidine + tryptophan  250 colonies

histidine + leucine         50 colonies 

leucine + tryptophan    500 colonies

histidine                       10 colonies

What is the order of transfer of the genes

TLS Online TPP Program

#Question id: 5279

#Unit 8. Inheritance Biology

In a transduction experiment, phage P1 is grown on a bacterial host of genotype A+ B+ C+ and the resulting lysate is used to infect a recipient strain of genotype A– B– C–. The genes are in an order such that B is in the middle, and the distance between A and B is greater than the distance between B and C. Based on this information, which of the following least genotype?

TLS Online TPP Program

#Question id: 5278

#Unit 8. Inheritance Biology

What is the co-transduction frequency for the A and B genes, from the following dataset?(Assume that there has been selection for the A+ form of the A gene). Genotype                                Number

 A+B+C+                                   10

 A+B+C-                                    30

A+B-C+                                     20

 A+B-C-                                     40

TLS Online TPP Program

#Question id: 5277

#Unit 8. Inheritance Biology

Deduce the genotype of bacteria strain was shown either growth presents or not in different media?

Media supplement

Minimal

Minimal + Arginine

Minimal + Methionine

Minimal + Arginine+ Methionine

Growth

No

Yes

     No

Yes

TLS Online TPP Program

#Question id: 5276

#Unit 8. Inheritance Biology

Donor Strain ABCDE transformation to   recipient strain abcde. Co-transformation check by using media with different supplement?

Media supplement

Growth

A

B

C

D

E

Yes

-

+

-

+

+

Yes

+

+

-

-

+

Yes

+

-

+

-

+

Yes

+

-

+

+

-

Yes

What is a order of gene ?

TLS Online TPP Program

#Question id: 5275

#Unit 8. Inheritance Biology

 The designation his- refers to