Practice Questions

TLS Online TPP Program

#Question id: 20284

What is the importance of C-peptide in proinsulin?

#Unit 7. System Physiology – Animal

It plays an important role in the correct folding of insulin and the formation of disulfide bridge
Because proinsulin and C peptide have virtually insulin activity
Patients with type 1 diabetes who are unable to produce insulin will usually have greatly increased levels of C peptide.
It elicits activation of at least one of these enzyme systems, sodium-potassium ATPase or endothelial nitric oxide synthase.

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TLS Online TPP Program

#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

TLS Online TPP Program

#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.

TLS Online TPP Program

#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___

TLS Online TPP Program

#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I

COLUMN II

A) Enhance efficiency

i) Multiplex PCR

B) Sequence specific amplification

ii) Hot-start PCR

C) Mutational analysis

iii) Megaprimer PCR

iv) qPCR with SYBR

v) AS-PCR

vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?

TLS Online TPP Program

#Question id: 19867

#Unit 13. Methods in Biology

Which of the following variants of PCR were devised to prevent non-specific amplification at low temperature?