DNA from a strain of Bacillus subtilis with genotype a+b+ c+d+ e+ is used to transform a strain with genotype a - b- c- d-   e-. Pairs of genes are checked for co-transformation and the following results are obtained:

Pair of co-transform genes as follow

D-E,             E-C,                C-B,                  A-D

On the basis of these results, what is the order of the genes on the bacterial chromosome?

#Question id: 537

#Unit 1. Molecules and their Interaction Relevant to Biology

Substrate level phosphorylation

A) describes the conversion of ADP into ATP with the addition of inorganic phosphate every place throughout the cell.

B) describes the formation of ADP by phosphoryl group transfer from 1,3 bisphosphoglycerate.

C) is the formation of ATP by phosphoryl group transfer from a higher energy compound.

#Question id: 536

#Unit 1. Molecules and their Interaction Relevant to Biology

Glucose 6-phosphate allosterically inhibits

A) hexokinase I.

B) glucokinase.

C) hexokinase II.

#Question id: 535

#Unit 1. Molecules and their Interaction Relevant to Biology

In some diabetic patients, glucose increases disproportionately and is unresponsive to an insulin challenge; under these conditions, how would oneʹs liver normally respond?

A) phosphorylating glucose for entry into the glycolytic pathway

B) saturating glucokinase with glucose

C) phosphorylating glucose for entry into the glycogen synthesis pathways

#Question id: 534

#Unit 1. Molecules and their Interaction Relevant to Biology

If glucose labeled with 14C in C-3 is metabolized to lactate via fermentation, the lactate will contain 14C in:

#Question id: 533

#Unit 1. Molecules and their Interaction Relevant to Biology

Glucose, labeled with 14C in different carbon atoms, is added to a crude extract of a tissue rich in the enzymes of the pentose phosphate pathway.  The most rapid production of 14CO2 will occur when the glucose is labeled in:

#Question id: 532

#Unit 1. Molecules and their Interaction Relevant to Biology

In glycolysis, fructose 1,6-bisphosphate is converted to two products with a standard free-energy change (DG'°) of 23.8 kJ/mol.  Under what conditions (encountered in a normal cell) will the free-energy change (DG) be negative, enabling the reaction to proceed to the right?