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TLS Online TPP Program

#Question id: 9151

What would be the effect on climate in the temperate latitudes if Earth were to slow its rate of rotation from a 24-hour period of rotation to a 48-hour period of rotation?

#Unit 9. Diversity of Life Forms

  1. Seasons would be longer and more distinct (colder winters and warmer summers).

  2. Large-scale weather events such as tornadoes and hurricanes would no longer be a part of regional climates.

  3. Winter seasons in both the northern and southern hemispheres would have more abundant and frequent precipitation events.

  4. There often would be a larger range between daytime high and nighttime low temperatures.

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TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than 
TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.
1. YFP
2. RFP
3. GFP
4. CFP
Which option below contains correct combination of flurorescent molecules that can give FRET?
TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as
1.  Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?