TLS Online TPP Program

#Question id: 12084


In bottom sediments of lakes and streams, organic matter is broken down by heterotrophic microorganisms and ammonia is released. Under aerobic conditions, specialized bacteria convert ammonia to nitrate (nitrification). This nitrate, together with nitrate from other sources, diffuses into the deeper sediments, where it may undergo anaerobic conversion to nitrogen gas (denitrification). These sediments typically contain oligochaete worms that live with their heads buried and their tails waving back and forth in the overlying water. Plastic columns were packed with freshly collected  stream  sediments  and  then  covered  with  layers  of  nitrate enriched  water.  A  similar  set  of  columns  was packed with sediment that had been sterilized and then covered with either nitrate-enriched water or distilled water. Oligochaete  worms  were  collected  and  acclimated  to  20°(C)Following  acclimation,  worms  were  rinsed  in  distilled water and then added to three sediment columns. The columns were incubated in the dark at 20°C and monitored every three days for changes in the concentration of nitrate in the overlying water. Nitrate concentrations in each of the experimental treatments were plotted against time, as shown in the graph below.


The best explanation for the increase in nitrate concentration in one treatment is that

#Unit 10. Ecological Principles
  1. Oligochaete worms may harbor microorganisms that are capable of carrying out nitrification
  2. Oligochaete worms are capable of carrying out a limited amount of denitrification in the absence of sediment microorganisms
  3. Some nitrifying bacteria must have survived the autoclaving in the form of spores
  4. Some denitrifying bacteria may have been added10to the columns along with the oligochaete worms

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TLS Online TPP Program

#Question id: 11066

#Unit 7. System Physiology – Animal

A 22-year-old woman has a pulmonary compliance of 0.2 L/cm H2O and a pleural pressure of −4 cm H2O. What is the pleural pressure (in cm H2O) when the woman inhales 1.0 L of air?

TLS Online TPP Program

#Question id: 909

#Unit 1. Molecules and their Interaction Relevant to Biology

Most proteins denature at both high and low pH. At high pH, the ionization of internal tyrosines is thought to be the main destabilizing influence, whereas at low pH, the protonation of buried histidines. A titration curve for the unfolding of the enzyme ribonuclease is shown in Figure. Given the relation between the titration curves for protein unfolding and histidine protonation, which of the following conclusion can be drawn correctly?

A. The sharp transition in graph indicates a highly cooperative process; when the protein starts to unfold, it completes the process rapidly

B. The sharp transition in graph indicates a highly noncooperative process

C. Several buried histidines can ionize when the chain starts to unfold, so that when one goes, they all go together due to cooperative behaviour of protein folding

D. Buried histidine (pK of 4 in this example) becomes accessible to solvent, its pK will shift toward its normal value of 6, significantly steepening its titration curve.

TLS Online TPP Program

#Question id: 12019

#Unit 10. Ecological Principles

Chickadees were given an opportunity to store seeds in 72 possible storage sites consisting of holes drilled in small trees that had been placed in an aviary. Typically, the seeds were placed in only 4 or 5 storage sites. The chickadees were then removed from the aviary, the seeds removed from the storage sites, and each hole covered. A day later, the chickadees were returned to the aviary and they spent nearly 5 times as long pulling at the covers on storage sites than at the covers on sites where they had not stored food. This experiment indicates that chickadees

TLS Online TPP Program

#Question id: 10352

#Unit 6. System Physiology – Plant

which of the following amino acid that participates in the malate–aspartate shuttle to transfer reducing equivalents from the mitochondrion and chloroplast into the cytosol?

TLS Online TPP Program

#Question id: 3415

#Unit 11. Evolution and Behavior

Imagine a plant of extremely variable height that is pollinated by three different pollinators, one that was attracted to short plants, another that preferred plants of medium height and a third that visited only the tallest plants. If the pollinator that preferred plants of medium height disappeared from an area. What is concluding of this condition?