The horizontal axis of the cladogram depicted below is a timeline that extends from 100,000 years ago to the present; the vertical axis represents nothing in particular. The labeled branch points on the tree (V-Z) represent various common ancestors. Let's say that only since 50,000 years ago has there been enough variation between the lineages depicted here to separate them into distinct species, and only the tips of the lineages on this tree represent distinct species.

 Which pair of scientists below would probably have agreed with the process that is depicted by this tree?

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#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 19787

#Unit 13. Methods in Biology

Polymerase chain reaction is based on___

a) Thermal modulation

b) pH dependent

c) Formation of amplicons by inserting some inputs for analysing its efficiency

d) Inter-complementary primers formation

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#Question id: 19186

#Unit 13. Methods in Biology

In another method of quantitative PCR, reporter-quencher set up is used. Which of the statement holds true for this methodology?

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#Question id: 19185

#Unit 13. Methods in Biology

Which of the statement hold true for Quantitative PCR?

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#Question id: 19180

#Unit 13. Methods in Biology

If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

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#Question id: 19178

#Unit 13. Methods in Biology

Touch-down PCR is another modification. Its characteristics include: