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TLS Online TPP Program

#Question id: 16966

CCR5 that plays a role in inflammatory response. Unfortunately, this protein is also a key to human immunodeficiency virus (HIV) infection. The virus binds to a loop of the CCR5 protein and then enters the cell. Some people have an allele called Δ32 at the CCR5 locus in which 32 bp are deleted. The missing part of the gene codes for the loop in the protein to which the virus attaches. Which statements is true for CCR5?

#Unit 11. Evolution and Behavior
  1. both homozygous and heterozygous partly resistant to HIV
  2. People who are homozygous for the deletion are partly resistant to HIV, while highly heterozygotes are resistant.
  3. People who are homozygous for the deletion are highly resistant to HIV, while heterozygotes are partly resistant.
  4. both homozygous and heterozygous highly resistant to HIV
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TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?
1.  It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

 Column I Column II 
 1. Texas a. far red
 2. Fluorescein b. red
 3. Rhodamine c. blue
 4. Cy5 d. green
Which of the following option comprises all correct matches?
TLS Online TPP Program

#Question id: 23155

#Unit 13. Methods in Biology

Match the following ions in column I with their relevant dyes column II.

 Column I Column II
 1. K+ a. Fura-2
 2. Ca2+ b. SNARF-1
 3. H+ c. Ion potassium green 
Which of the following options is correct?
TLS Online TPP Program

#Question id: 23152

#Unit 13. Methods in Biology

A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of 
1. Alkaline cells
2. Acidic cells 
3. Dead cells
4. Live cells
Which of the following options are incorrect?