Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 380

#Unit 1. Molecules and their Interaction Relevant to Biology

The Henderson-Hasselbalch equation:

TLS Online TPP Program

#Question id: 4118

#Unit 3. Fundamental Processes

If the 5’ splice site sequence changed from 5’- GUAAGU-3’ to 5’-GUAUGU-3’, predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein– RNA binding assay.

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#Question id: 931

#Unit 1. Molecules and their Interaction Relevant to Biology

The major site of formation of acetoacetate from fatty acids is the:

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#Question id: 15382

#Unit 8. Inheritance Biology

In a group of fruit flies homozygous for an allele that causes a reduced number of thoracic bristles, you notice that the number of bristles varies from about half the normal number to only a few bristles. This is an example of which of the following?

TLS Online TPP Program

#Question id: 12185

#Unit 2. Cellular Organization

Mitotic CDKs affect nuclear envelope components by specific mechanism. Which of the following statement is not related to this mechanism?