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TLS Online TPP Program
#Question id: 3880
#SCPH01 Biochemistry
Which one of the following properties of the L-19 IVS ribozyme is not shared with enzymes that are purely protein?
TLS Online TPP Program
#Question id: 13093
#SCPH05 I Biotechnology
You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?
In this table, DV = digested vector. DYG = digested yeast genome.
TLS Online TPP Program
#Question id: 4173
#SCPH28 | Zoology
Different codons that specify the same amino acid are called __ codons.
TLS Online TPP Program
#Question id: 451
#SCPH05 I Biotechnology
The standard reduction potentials (E'°) for the following half reactions are given.
Fumarate + 2H+ + 2e– -> succinate E'° = +0.031 V
FAD + 2H+ + 2e– -> FADH2 E'° = –0.219 V
If you mixed succinate, fumarate, FAD, and FADH2 together, all at l M concentrations and in the presence of succinate dehydrogenase, which of the following would happen initially?
TLS Online TPP Program
#Question id: 12675
#SCPH06 I Botany
Use the incomplete diagram below, illustrating some of the steps involved in eutrophication to answer the following questions
What would be a likely entry for box A?