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#Question id: 2105

An investigator wants to use FRAP to quantify the lateral movement of a specific plasma membrane protein, but to do so this investigator must first:

#I Life Science/ Life Sciences Group – I-V

  1. focus a laser on one region of the cell surface.

  2. measures the intensity of the fluorescence of the cell surface.

  3. label the cell with a fluorescent reagent that binds specifically to the cell surface.

  4. add detergents to make the cell surface more fluid.

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TLS Online TPP Program

#Question id: 14774

#SCPH06 I Botany

A problem in mathematics is given to three students Dayanand, Ramesh and Naresh whose chances of solving it are 1/2, 1/3, 1/4 respectively. The probability that the problem is solved is
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#Question id: 16130

#SCPH06 I Botany

You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.              
How many units of ß-galactiosidase would you expect to be expressed from deletion 1 in a gln2– mutant? 
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#Question id: 13081

#SCPH05 I Biotechnology

Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
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#Question id: 67

#SCPH06 I Botany

When a region of DNA must be repaired by removing and replacing some of the nucleotides, what ensures that the new nucleotides are in the correct sequence?
TLS Online TPP Program

#Question id: 344

#SCPH05 I Biotechnology

The partial negative charge at one end of a water molecule is attracted to the partial positive charge of another water molecule. What is this attraction called?