#Question id: 27186
#SCPH05 I Biotechnology
#Question id: 13076
#SCPH05 I Biotechnology
Restriction fragment length polymorphism denotes that a
single restriction enzyme produces fragments of different lengths from the same
stretch of genomic DNA of different strains of a species or from different
related species. RFLPs are detected as follows:
i. Large
molecular weight genomic DNA is isolated from several strains or related
species;
ii. The
fragments in these digests are separated through electrophoresis
iii. These
'DNAs are then digested with a selected restriction enzyme
iv. Exposed
to a suitably radio-labelled appropriate DNA probe under conditions favouring
DNA: DNA hybridization
v. The
resulting gel lanes are transferred and fixed to a suitable solid support and
vi. The free
probes are removed
vii. The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
#Question id: 35
#SCPH28 | Zoology
Arrange the following molecular components of an E. coli cell in terms of their percentage of total weight cell.
#Question id: 1775
#SCPH01 Biochemistry
How many CDR region in IgM ?
#Question id: 14497
#SCPH01 Biochemistry