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#Question id: 335
#SCPH05 I Biotechnology
Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25 °C) to prevent swelling and lysis?
TLS Online TPP Program
#Question id: 15618
#SCPH28 | Zoology
Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition isolate a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for Kanr transductants. In this case, all 100 Kanr transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?
TLS Online TPP Program
#Question id: 17074
#SCPH01 Biochemistry
What are some disadvantages of monoclonal antibody production?
TLS Online TPP Program
#Question id: 23200
#SCPH06 I Botany
Scanning electron microscopy (SEM) allows investigators to view the
TLS Online TPP Program
#Question id: 9216
#SCPH28 | Zoology
Which of the following criteria have to be met for a species to qualify as invasive?