TLS Online TPP Program

#Question id: 2627


Binding of repressor on to the operator sequences, RNA polymerase binding is analyzed by; Which one is incorrect.

#I Life Science/ Life Sciences Group – I-V
  1. DNA-footprinting

  2. Gel-mobility assays

  3. EMSA

  4. Only A & B

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TLS Online TPP Program

#Question id: 442

#SCPH05 I Biotechnology

Levels of ATP and ADP are partly maintained in cells by

TLS Online TPP Program

#Question id: 12927

#SCPH01 Biochemistry

When the ends cut by enzyme are not only identical but complementary and will base pair with each other they are therefore known as _______

TLS Online TPP Program

#Question id: 789

#SCPH05 I Biotechnology

Presence of large numbers of modified specially methylated nucleotides in tRNA contribute in

TLS Online TPP Program

#Question id: 12738

#SCPH06 I Botany

Match  the following:
1. Growth Factors                                                    i. GTPase
2. Protein kinases                                                    ii. PDGF
3. G- Proteins                                                           iii. DNA binding protein
4. Nucleus located transcription factors      iv.Serine Kinases

TLS Online TPP Program

#Question id: 10284

#I Life Science/ Life Sciences Group – I-V

Allosteric regulation of enzyme multisubunit or multiactive site shows cooperativity with the sigmoidal graph between substerate conc. v/s Activity,


a) Cell contain NADH, FADH2 and ATP, that inhibits the allosteric enzyme activity by lowering the affinity with its substate, graph shifts towards B from control

 b) A- shows low ATP, the Km for fructose 6-phosphate is relatively low, the affinity of the enzyme with its substate is relatively high

 c) Haemoglobin, Phosphofructokinase, Phosphate dehydrogenase complex, α-ketoglutarate dehydrogenase complex, these all enzymes shows characteristics of sigmoidal graph

 d) When ATP is high, Km for fructose 6-phosphate is greatly increased- this activity shown by B in the graph

 e) fructose 2,6-bisphosphate binds to its allosteric site on PFK-1, it increases the enzyme’s affinity for its substrate fructose 6-phosphate and reduces its affinity for the allosteric inhibitors ATP and citrate- shown in graph by A

 Which of the following  combination of the enzyme activity with its substate correctly shown;